LOCUS       V008100                 6690 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008100
VERSION     V008100
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Akagi T, Sasai K, Hanafusa H.
  TITLE     Refractory nature of normal human diploid fibroblasts with respect
            to oncogene-mediated transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 100 (23), 13567-13572 (2003)
   PUBMED   14597713
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute,
            Molecular Oncology; Furuedai 6-2-4, Suita, Osaka 565-0874, Japan
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax:81-6-6871-7521)
REFERENCE   3  (bases 1 to 6690)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2003"; volume: "100"; issue: "23"; pages:
            "13567-13572"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute, Molecular
            Oncology"; volume: " Furuedai 6-2-4, Suita, Osaka 565-0874, Japan
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax"; pages:
            "81-6-6871-7521"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label="R-U5 region of MuLV 5' LTR"
                     /note="R-U5 region of MuLV 5' LTR"
     misc_feature    826..1183
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1242..1658
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1668..2018
                     /label="Mo-MuLV pol region containing splice acceptor"
                     /note="Mo-MuLV pol region containing splice acceptor"
     misc_feature    2019..2080
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    2128..2689
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2692..3993
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Salmonella typhimurium
                     (strain LT2 / SGSC1412 / ATCC 700720). Accession#: P10370"
     LTR             4047..4640
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4877..5465)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5632..6489)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6490..6594)
                     /label="AmpR promoter"
ORIGIN
        1 tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg
       61 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
      121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta gagaaccatc
      481 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
      601 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
      661 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
      721 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
      781 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
      961 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     1021 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat
     1081 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     1141 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     1201 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     1261 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1321 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1381 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1441 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1501 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1561 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1621 cctcccttta tccagccctc actccttctc taggcgcccc catatggcca tatgagatct
     1681 tatatggggc acccccgccc cttgtaaact tccctgaccc tgacttgaca agagttacta
     1741 acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga
     1801 gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc
     1861 gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga
     1921 aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag
     1981 cttggataca cgccgcccac gtgaaggctg ccgaccccgg atcccagtgt ggtggtacgg
     2041 gaattccgga ccgacgcgtg cggccgcggt aacaattgtt aactaactta agctagcaac
     2101 ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc
     2161 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     2221 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     2281 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     2341 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     2401 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     2461 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     2521 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     2581 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag
     2641 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catgagcttc
     2701 aataccctga ttgactggaa cagctgtagc cctgaacagc agcgtgcgct gctgacgcgt
     2761 ccggcgattt ccgcctctga cagtattacc cggacggtca gcgatattct ggataatgta
     2821 aaaacgcgcg gtgacgatgc cctgcgtgaa tacagcgcta aatttgataa aacagaagtg
     2881 acagcgctac gcgtcacccc tgaagagatc gccgccgccg gcgcgcgtct gagcgacgaa
     2941 ttaaaacagg cgatgaccgc tgccgtcaaa aatattgaaa cgttccattc cgcgcagacg
     3001 ctaccgcctg tagatgtgga aacccagcca ggcgtgcgtt gccagcaggt tacgcgtccc
     3061 gtctcgtctg tcggtctgta tattcccggc ggctcggctc cgctcttctc aacggtgctg
     3121 atgctggcga cgccggcgcg cattgcggga tgccagaagg tggttctgtg ctcgccgccg
     3181 cccatcgctg atgaaatcct ctatgcggcg caactgtgtg gcgtgcagga aatctttaac
     3241 gtcggcggcg cgcaggcgat tgccgctctg gccttcggca gcgagtccgt accgaaagtg
     3301 gataaaattt ttggccccgg caacgccttt gtaaccgaag ccaaacgtca ggtcagccag
     3361 cgtctcgacg gcgcggctat cgatatgcca gccgggccgt ctgaagtact ggtgatcgca
     3421 gacagcggcg caacaccgga tttcgtcgct tctgacctgc tctcccaggc tgagcacggc
     3481 ccggattccc aggtgatcct gctgacgcct gatgctgaca ttgcccgcaa ggtggcggag
     3541 gcggtagaac gtcaactggc ggaactgccg cgcgcggaca ccgcccggca ggccctgagc
     3601 gccagtcgtc tgattgtgac caaagattta gcgcagtgcg tcgccatctc taatcagtat
     3661 gggccggaac acttaatcat ccagacgcgc aatgcgcgcg atttggtgga tgcgattacc
     3721 agcgcaggct cggtatttct cggcgactgg tcgccggaat ccgccggtga ttacgcttcc
     3781 ggaaccaacc atgttttacc gacctatggc tatactgcta cctgttccag ccttgggtta
     3841 gcggatttcc agaaacggat gaccgttcag gaactgtcga aagcgggctt ttccgctctg
     3901 gcatcaacca ttgaaacatt ggcggcggca gaacgtctga ccgcccataa aaatgccgtg
     3961 accctgcgcg taaacgccct caaggagcaa gcatgagtcg acacgataaa ataaaagatt
     4021 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     4081 gcttaagtaa cgccattttg gaaggcatgg aaaaatacat aactgagaat agagaagttc
     4141 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     4201 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     4261 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     4321 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     4381 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     4441 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     4501 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     4561 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     4621 gtcagcgggg gtctttcatt ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4681 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5281 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
     5581 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5641 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5701 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5761 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5821 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5881 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5941 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6001 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6061 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6121 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6181 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6241 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6301 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6361 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6421 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6481 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6541 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6601 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6661 aaataggcgt atcacgaggc cctttcgtcc
//
